dc.contributor.authorZhu, Detu
dc.contributor.authorZhao, Zuxianglan
dc.contributor.authorCui, Guimei
dc.contributor.authorChang, Shiehong
dc.contributor.authorHu, Lingling
dc.contributor.authorSee, Yi Xiang
dc.contributor.authorLim, Michelle Gek Liang
dc.contributor.authorGuo, Dajiang
dc.contributor.authorChen, Xin
dc.contributor.authorRobson, Paul
dc.contributor.authorLuo, Yumei
dc.contributor.authorCheung, Edwin
dc.identifier.citationZhu, D., Zhao, Z., Cui, G., Chang, S., Hu, L., See, Y. X., . . . Cheung, E. (2018). Single-cell transcriptome analysis reveals estrogen signaling coordinately augments one-carbon, polyamine, and purine synthesis in breast cancer. Cell Reports, 25(8), 2285-2298. doi:10.1016/j.celrep.2018.10.093en_US
dc.description.abstractEstrogen drives breast cancer (BCa) progression by directly activating estrogen receptor α (ERα). However, because of the stochastic nature of gene transcription, it is important to study the estrogen signaling pathway at the single-cell level to fully understand how ERα regulates transcription. Here, we performed single-cell transcriptome analysis on ERα-positive BCa cells following 17β-estradiol stimulation and reconstructed the dynamic estrogen-responsive transcriptional network from discrete time points into a pseudotemporal continuum. Notably, differentially expressed genes show an estrogen-stimulated metabolic switch that favors biosynthesis but reduces estrogen degradation. Moreover, folate-mediated one-carbon metabolism is reprogrammed through the mitochondrial folate pathway and polyamine and purine synthesis are upregulated coordinately. Finally, we show AZIN1 and PPAT are direct ERα targets that are essential for BCa cell survival and growth. In summary, our study highlights the dynamic transcriptional heterogeneity in ERα-positive BCa cells upon estrogen stimulation and uncovers a mechanism of estrogen-mediated metabolic switch.en_US
dc.format.extent33 p.en_US
dc.relation.ispartofseriesCell Reportsen_US
dc.rights© 2018 The Authors. (Published by Elsevier). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.subjectBreast Canceren_US
dc.subjectSingle-Cell Transcriptomeen_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleSingle-cell transcriptome analysis reveals estrogen signaling coordinately augments one-carbon, polyamine, and purine synthesis in breast canceren_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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