dc.contributor.authorPotroz, Michael G.
dc.contributor.authorMundargi, Raghavendra C.
dc.contributor.authorPark, Jae Hyeon
dc.contributor.authorTan, Ee-Lin
dc.contributor.authorCho, Nam-joon
dc.identifier.citationPotroz, M. G., Mundargi, R. C., Park, J. H., Tan, E.-L., & Cho, N.-j. (2016). Extraction of plant-based capsules for microencapsulation applications. Journal of Visualized Experiments, (117), e54768-. doi:10.3791/54768en_US
dc.description.abstractMicrocapsules derived from plant-based spores or pollen provide a robust platform for a diverse range of microencapsulation applications. Sporopollenin exine capsules (SECs) are obtained when spores or pollen are processed so as to remove the internal sporoplasmic contents. The resulting hollow microcapsules exhibit a high degree of micromeritic uniformity and retain intricate microstructural features related to the particular plant species. Herein, we demonstrate a streamlined process for the production of SECs from Lycopodium clavatum spores and for the loading of hydrophilic compounds into these SECs. The current SEC isolation procedure has been recently optimized to significantly reduce the processing requirements which are conventionally used in SEC isolation, and to ensure the production of intact microcapsules. Natural L. clavatum spores are defatted with acetone, treated with phosphoric acid, and extensively washed to remove sporoplasmic contents. After acetone defatting, a single processing step using 85% phosphoric acid has been shown to remove all sporoplasmic contents. By limiting the acid processing time to 30 hr, it is possible to isolate clean SECs and avoid SEC fracturing, which has been shown to occur with prolonged processing time. Extensive washing with water, dilute acids, dilute bases, and solvents ensures that all sporoplasmic material and chemical residues are adequately removed. The vacuum loading technique is utilized to load a model protein (Bovine Serum Albumin) as a representative hydrophilic compound. Vacuum loading provides a simple technique to load various compounds without the need for harsh solvents or undesirable chemicals which are often required in other microencapsulation protocols. Based on these isolation and loading protocols, SECs provide a promising material for use in a diverse range of microencapsulation applications, such as, therapeutics, foods, cosmetics, and personal care products.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent10 p.en_US
dc.relation.ispartofseriesJournal of Visualized Experimentsen_US
dc.rights© 2016 Journal of Visualized Experiments. All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of Journal of Visualized Experiments.en_US
dc.subjectLycopodium clavatumen_US
dc.titleExtraction of plant-based capsules for microencapsulation applicationsen_US
dc.typeJournal Article
dc.contributor.researchCentre for Biomimetic Sensor Scienceen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.versionPublished versionen_US

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