Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/81564
Title: | Inducing ischemia-reperfusion injury in the mouse ear skin for intravital multiphoton imaging of immune responses | Authors: | Goh, Chi Ching Li, Jackson LiangYao Becker, David Weninger, Wolfgang Angeli, Veronique Ng, Lai Guan |
Keywords: | Medicine DRNTU::Science::Medicine Immunology |
Issue Date: | 2016 | Source: | Goh, C. C., Li, J. L., Becker, D., Weninger, W., Angeli, V., & Ng, L. G. (2016). Inducing ischemia-reperfusion injury in the mouse ear skin for intravital multiphoton imaging of immune responses, (118), e54956-. doi:10.3791/54956 | Series/Report no.: | Journal of Visualized Experiments | Abstract: | Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent re-oxygenation of the tissues (reperfusion). In the skin, ischemia-reperfusion (IR) is the main contributing factor to the pathophysiology of pressure ulcers. While the cascade of events leading up to the inflammatory response has been well studied, the spatial and temporal responses of the different subsets of immune cells to an IR injury are not well understood. Existing models of IR using the clamping technique on the skin flank are highly invasive and unsuitable for studying immune responses to injury, while similar non-invasive magnet clamping studies in the skin flank are less-than-ideal for intravital imaging studies. In this protocol, we describe a robust model of non-invasive IR developed on mouse ear skin, where we aim to visualize in real-time the cellular response of immune cells after reperfusion via multiphoton intravital imaging (MP-IVM). | URI: | https://hdl.handle.net/10356/81564 http://hdl.handle.net/10220/47496 |
ISSN: | 1940-087X | DOI: | 10.3791/54956 | Schools: | School of Biological Sciences Lee Kong Chian School of Medicine (LKCMedicine) |
Rights: | © 2016 Journal of Visualized Experiments. All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of Journal of Visualized Experiments. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | LKCMedicine Journal Articles SBS Journal Articles |
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Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses.pdf | 1.05 MB | Adobe PDF | View/Open |
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