dc.contributor.authorShi, Meng
dc.contributor.authorChen, Bing
dc.contributor.authorMahajan, Divyanshu
dc.contributor.authorBoh, Boon Kim
dc.contributor.authorZhou, Yan
dc.contributor.authorDutta, Bamaprasad
dc.contributor.authorTie, Hieng Chiong
dc.contributor.authorSze, Siu Kwan
dc.contributor.authorWu, Geng
dc.contributor.authorLu, Lei
dc.identifier.citationShi, M., Chen, B., Mahajan, D., Boh, B. K., Zhou, Y., Dutta, B., . . . Lu, L. (2018). Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nature Communications, 9(1), 4987-. doi:10.1038/s41467-018-07444-yen_US
dc.description.abstractThe endosome-to-Golgi or endocytic retrograde trafficking pathway is an important post-Golgi recycling route. Here we show that amino acids (AAs) can stimulate the retrograde trafficking and regulate the cell surface localization of certain Golgi membrane proteins. By testing components of the AA-stimulated mTORC1 signaling pathway, we demonstrate that SLC38A9, v-ATPase and Ragulator, but not Rag GTPases and mTORC1, are essential for the AA-stimulated trafficking. Arl5, an ARF-like family small GTPase, interacts with Ragulator in an AA-regulated manner and both Arl5 and its effector, the Golgi-associated retrograde protein complex (GARP), are required for the AA-stimulated trafficking. We have therefore identified a mechanistic connection between the nutrient signaling and the retrograde trafficking pathway, whereby SLC38A9 and v-ATPase sense AA-sufficiency and Ragulator might function as a guanine nucleotide exchange factor to activate Arl5, which, together with GARP, a tethering factor, probably facilitates the endosome-to-Golgi trafficking.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent16 p.en_US
dc.relation.ispartofseriesNature Communicationsen_US
dc.rights© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.subjectSmall GTPaseen_US
dc.titleAmino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5en_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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