dc.contributor.authorWunder, Tobias
dc.contributor.authorCheng, Steven Le Hung
dc.contributor.authorLai, Soak-Kuan
dc.contributor.authorLi, Hoi-Yeung
dc.contributor.authorMueller-Cajar, Oliver
dc.identifier.citationWunder, T., Cheng, S. L. H., Lai, S.-K., Li, H.-Y., & Mueller-Cajar, O. (2018). The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger. Nature Communications, 9(1), 5076-. doi:10.1038/s41467-018-07624-wen_US
dc.description.abstractThe slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membranous compartment of the chloroplast stroma. Here we show using pure components that two proteins, Rubisco and the linker protein Essential Pyrenoid Component 1 (EPYC1), are both necessary and sufficient to phase separate and form liquid droplets. The phase-separated Rubisco is functional. Droplet composition is dynamic and components rapidly exchange with the bulk solution. Heterologous and chimeric Rubiscos exhibit variability in their tendency to demix with EPYC1. The ability to dissect aspects of pyrenoid biochemistry in vitro will permit us to inform and guide synthetic biology ambitions aiming to engineer microalgal CCMs into crop plants.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent10 p.en_US
dc.relation.ispartofseriesNature Communicationsen_US
dc.rights© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.subjectEssential Pyrenoid Component 1en_US
dc.titleThe phase separation underlying the pyrenoid-based microalgal Rubisco superchargeren_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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