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Title: Human rett-derived neuronal progenitor cells in 3D graphene scaffold as an in vitro platform to study the effect of electrical stimulation on neuronal differentiation
Authors: Nguyen, Anh Tuan
Mattiassi, Sabrina
Loeblein, Manuela
Chin, Eunice
Ma, DongLiang
Coquet, Philippe
Viasnoff, Virgile
Teo, Edwin Hang Tong
Goh, Eyleen L.
Yim, Evelyn K. F.
Keywords: DRNTU::Engineering::Electrical and electronic engineering
Deep Brain Stimulation
Rett Syndrome
Issue Date: 2018
Source: Nguyen, A. T., Mattiassi, S., Loeblein, M., Chin, E., Ma, D., Coquet, P., . . . Yim, E. K. F. (2018). Human Rett-derived neuronal progenitor cells in 3D graphene scaffold as an in vitro platform to study the effect of electrical stimulation on neuronal differentiation. Biomedical Materials, 13(3), 034111-. doi:10.1088/1748-605X/aaaf2b
Series/Report no.: Biomedical Materials
Abstract: Studies of electrical stimulation therapies for the treatment of neurological disorders, such as deep brain stimulation, have almost exclusively been performed using animal-models. However, because animal-models can only approximate human brain disorders, these studies should be supplemented with an in vitro human cell-culture based model to substantiate the results of animal-based studies and further investigate therapeutic benefit in humans. This study presents a novel approach to analyze the effect of electrical stimulation on the neurogenesis of patient-induced pluripotent stem cell (iPSC) derived neural progenitor cell (NPC) lines, in vitro using a 3D graphene scaffold system. The iPSC-derived hNPCs used to demonstrate the system were collected from patients with Rett syndrome, a debilitating neurodevelopmental disorder. The graphene scaffold readily supported both the wild-type and Rett NPCs. Electrical stimulation parameters were optimized to accommodate both wild-type and Rett cells. Increased cell maturation and improvements in cell morphology of the Rett cells was observed after electrical stimulation. The results of the pilot study of electrical stimulation to enhance Rett NPCs neurogenesis were promising and support further investigation of the therapy. Overall, this system provides a valuable tool to study electrical stimulation as a potential therapy for neurological disorders using patient-specific cells.
ISSN: 1748-6041
DOI: 10.1088/1748-605X/aaaf2b
Rights: © 2018 IOP Publishing Ltd. All rights reserved. This is an author-created, un-copyedited version of an article accepted for publication in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The definitive publisher authenticated version is available online at
Fulltext Permission: open
Fulltext Availability: With Fulltext
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