Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/90116
Title: | Myocardial injury is distinguished from stable angina by a set of candidate plasma biomarkers identified using iTRAQ/MRM-based approach | Authors: | Cheow, Esther Sok Hwee Cheng, Woo Chin Yap, Terence Dutta, Bamaprasad Lee, Chuen Neng Kleijn, Dominique P. V. de Sorokin, Vitaly Sze, Siu Kwan |
Keywords: | Atherosclerosis DRNTU::Science::Biological sciences Cardiovascular Disease |
Issue Date: | 2017 | Source: | Cheow, E. S. H., Cheng, W. C., Yap, T., Dutta, B., Lee, C. N., Kleijn, D. P. V. d., . . . Sze, S. K. (2017). Myocardial Injury Is Distinguished from Stable Angina by a Set of Candidate Plasma Biomarkers Identified Using iTRAQ/MRM-Based Approach. Journal of Proteome Research, 17(1), 499-515. doi:10.1021/acs.jproteome.7b00651 | Series/Report no.: | Journal of Proteome Research | Abstract: | The lack of precise biomarkers that identify patients at risk for myocardial injury and stable angina delays administration of optimal therapy. Hence, the search for noninvasive biomarkers that could accurately stratify patients with impending heart attack, from patients with stable coronary artery disease (CAD), is urgently needed in the clinic. Herein, we performed comparative quantitative proteomics on whole plasma sampled from patients with stable angina (NMI), acute myocardial infarction (MI), and healthy control subjects (Ctrl). We detected a total of 371 proteins with high confidence (FDR < 1%, p < 0.05) including 53 preliminary biomarkers that displayed ≥2-fold modulated expression in patients with CAD (27 associated with atherosclerotic stable angina, 26 with myocardial injury). In the verification phase, we used label-free LC–MRM-MS-based targeted method to verify the preliminary biomarkers in pooled plasma, excluded peptides that were poorly distinguished from background, and performed further validation of the remaining candidates in 49 individual plasma samples. Using this approach, we identified a final panel of eight novel candidate biomarkers that were significantly modulated in CAD (p < 0.05) including proteins associated with atherosclerotic stable angina that were implicated in endothelial dysfunction (F10 and MST1), proteins associated with myocardial injury reportedly involved in plaque destabilization (SERPINA3, CPN2, LUM), and in tissue protection/repair mechanisms (ORM2, ACTG1, NAGLU). Taken together, our data showed that candidate biomarkers with potential diagnostic values can be successfully detected in nondepleted human plasma using an iTRAQ/MRM-based discovery-validation approach and demonstrated the plausible clinical utility of the proposed panel in discriminating atherosclerotic stable angina from myocardial injury in the studied cohort. | URI: | https://hdl.handle.net/10356/90116 http://hdl.handle.net/10220/48403 |
ISSN: | 1535-3893 | DOI: | 10.1021/acs.jproteome.7b00651 | Schools: | School of Biological Sciences | Rights: | © 2017 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.7b00651. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Myocardial Injury Is Distinguished from Stable Angina by a Set of Candidate Plasma Biomarkers Identified Using iTRAQ MRM-Based Approach.pdf | 1.13 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
20
18
Updated on Mar 27, 2024
Web of ScienceTM
Citations
20
18
Updated on Oct 28, 2023
Page view(s) 50
500
Updated on Mar 27, 2024
Download(s) 20
331
Updated on Mar 27, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.