Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/90151
Title: Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
Authors: Zhang, Hao
Li, Tianhu
Keywords: Nucleosome
Histone Octamer
DRNTU::Science::Chemistry
Issue Date: 2018
Source: Zhang, H., & Li, T. (2018). Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes. Bioorganic & Medicinal Chemistry Letters, 28(3), 537-540. doi:10.1016/j.bmcl.2017.10.072
Series/Report no.: Bioorganic & Medicinal Chemistry Letters
Abstract: With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on average, binding of ∼11.5 histone H1 molecules causes one linking number change in circular polynucleosomes in the presence of 1.5 mM spermidine. When concentrations of spermidine decreases or increases, these linking number differences decrease significantly. It is therefore evident that linking number changes caused by histone H1 are spermidine concentration-dependent.
URI: https://hdl.handle.net/10356/90151
http://hdl.handle.net/10220/48431
ISSN: 0960-894X
DOI: http://dx.doi.org/10.1016/j.bmcl.2017.10.072
Rights: © 2017 Elsevier Ltd.. All rights reserved. This paper was published in Bioorganic & Medicinal Chemistry Letters and is made available with permission of Elsevier Ltd.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles

Files in This Item:
File Description SizeFormat 
Quantitative determination of linking number differences between circula.._.pdf564.56 kBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.