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Title: The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell mass
Authors: Jahan, Mahabuba
Johnström, Peter
Selvaraju, Ram K.
Svedberg, Marie
Winzell, Maria Sörhede
Bernström, Jenny
Kingston, Lee
Schou, Magnus
Jia, Zhisheng
Skrtic, Stanko
Johansson, Lars
Korsgren, Olle
Farde, Lars
Halldin, Christer
Eriksson, Olof
Keywords: DRNTU::Science::Medicine
Beta Cell Imaging
G-Protein-Coupled Receptor 44 (GPR44)
Issue Date: 2018
Source: Jahan, M., Johnström, P., Selvaraju, R. K., Svedberg, M., Winzell, M. S., Bernström, J., . . . Eriksson, O. (2018). The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell mass. EJNMMI Research, 8, 113-. doi:10.1186/s13550-018-0465-6
Series/Report no.: EJNMMI Research
Abstract: Background: The G-protein-coupled receptor 44 (GPR44) is a beta cell-restricted target that may serve as a marker for beta cell mass (BCM) given the development of a suitable PET ligand. Methods: The binding characteristics of the selected candidate, AZ12204657, at human GPR44 were determined using in vitro ligand binding assays. AZ12204657 was radiolabeled using 11C- or 3H-labeled methyl iodide ([11C/3H]CH3I) in one step, and the conversion of [11C/3H]CH3I to the radiolabeled product [11C/3H]AZ12204657 was quantitative. The specificity of radioligand binding to GPR44 and the selectivity for beta cells were evaluated by in vitro binding studies on pancreatic sections from human and non-human primates as well as on homogenates from endocrine and exocrine pancreatic compartments. Results: The radiochemical purity of the resulting radioligand [11C]AZ12204657 was > 98%, with high molar activity (MA), 1351 ± 575 GBq/μmol (n = 18). The radiochemical purity of [3H]AZ12204657 was > 99% with MA of 2 GBq/μmol. Pancreatic binding of [11C/3H]AZ12204657 was co-localized with insulin-positive islets of Langerhans in non-diabetic individuals and individuals with type 2 diabetes (T2D). The binding of [11C]AZ12204657 to GPR44 was > 10 times higher in islet homogenates compared to exocrine homogenates. In human islets of Langerhans GPR44 was co-expressed with insulin, but not glucagon as assessed by co-staining and confocal microscopy. Conclusion: We radiolabeled [11C]AZ12204657, a potential PET radioligand for the beta cell-restricted protein GPR44. In vitro evaluation demonstrated that [3H]AZ12204657 and [11C]AZ12204657 selectively target pancreatic beta cells. [11C]AZ12204657 has promising properties as a marker for human BCM.
DOI: 10.1186/s13550-018-0465-6
Rights: © 2018 The Author(s). Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0International License (, which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link tothe Creative Commons license, and indicate if changes were made.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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