Pulsed laser diode based desktop photoacoustic tomography for monitoring wash-in and wash-out of dye in rat cortical vasculature
Kalva, Sandeep Kumar
Upputuri, Paul Kumar
Dienzo, Rhonnie Austria
Date of Issue2019
School of Chemical and Biomedical Engineering
Photoacoustic (PA) tomography (PAT) imaging is an emerging biomedical imaging modality useful in various preclinical and clinical applications. Custom-made circular ring array-based transducers and conventional bulky Nd:YAG/OPO lasers inhibit translation of the PAT system to clinics. Ultra-compact pulsed laser diodes (PLDs) are currently being used as an alternative source of near-infrared excitation for PA imaging. Highspeed dynamic in vivo imaging has been demonstrated using a compact PLD-based desktop PAT system (PLD-PAT). A visualized experimental protocol using the desktop PLD-PAT system is provided in this work for dynamic in vivo brain imaging. The protocol describes the desktop PLDPAT system configuration, preparation of animal for brain vascular imaging, and procedure for dynamic visualization of indocyanine green (ICG) dye uptake and clearance process in rat cortical vasculature.
Journal of Visualized Experiments
© 2019 The Author(s) (Journal of Visualized Experiments). All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of The Author(s) (Journal of Visualized Experiments).