Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/85760
Title: Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
Authors: Ye, Kehan
Sinawang, Prima Dewi
Tok, Alfred Iing Yoong
Marks, Robert S.
Keywords: Diazirine Silane
Photoinducible Crosslinker
Engineering::Materials
Issue Date: 2018
Source: Ye, K., Sinawang, P. D., Tok, A. I. Y., & Marks, R. S. (2018). Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 256, 234-242. doi:10.1016/j.snb.2017.10.058
Series/Report no.: Sensors and Actuators B: Chemical
Abstract: One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. After optimization, it has shown superior adaptability over traditional chemiluminescent ELISA and a commonly used glutaraldehyde chemical immobilization method in detection limit (6.44 * 102 CFU/ml) and dynamic range (6.44 *105 to 6.44 *108 CFU/ml, r2 = 0.98; 6.44 *102 to 6.44 *105, r2 = 0.92). The newly developed immobilization method has enhanced the function of FOBS as an affordable and dispatchable tool in the early warning monitoring of the putative presence of E. coli cells. It can be used either in individual sample screening or in continuous monitoring of the environment to indicate the need for further investigation. The diazirine crosslinking method can also be adopted in the immobilization of other protein-based bioentities thus expanding its real-world utility.
URI: https://hdl.handle.net/10356/85760
http://hdl.handle.net/10220/49245
ISSN: 0925-4005
DOI: 10.1016/j.snb.2017.10.058
Rights: © 2017 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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