Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/82615
Title: Near-infrared afterglow semiconducting nano-polycomplexes for multiplex differentiation of cancer exosomes
Authors: Lyu, Yan
Cui, Dong
Huang, Jiaguo
Fan, Wenxuan
Miao, Yansong
Pu, Kanyi
Keywords: Exosomes
Biosensors
Engineering::Chemical engineering
Issue Date: 2019
Source: Lyu, Y., Cui, D., Huang, J., Fan, W., Miao, Y., & Pu, K. (2019). Near-infrared afterglow semiconducting nano-polycomplexes for the multiplex differentiation of cancer exosomes. Angewandte Chemie International Edition, 58(15), 4983-4987. doi:10.1002/anie.201900092
Series/Report no.: Angewandte Chemie International Edition
Abstract: The detection of exosomes is promising for the early diagnosis of cancer. However, the development of suitable optical sensors remains challenging. We have developed the first luminescent nanosensor for the multiplex differentiation of cancer exosomes that bypasses real‐time light excitation. The sensor is composed of a near‐infrared semiconducting polyelectrolyte (ASPN) that forms a complex with a quencher‐tagged aptamer. The afterglow signal of the nanocomplex (ASPNC), being initially quenched, is turned on in the presence of aptamer‐targeted exosomes. Because detection of the afterglow takes place after the excitation, background signals are minimized, leading to an improved limit of detection that is nearly two orders of magnitude lower than that of fluorescence detection in cell culture media. Also, ASPNC can be easily tailored to detect different exosomal proteins by changing the aptamer sequence. This enables an orthogonal analysis of multiple exosome samples, potentially permitting an accurate identification of the cellular origin of exosomes for cancer diagnosis.
URI: https://hdl.handle.net/10356/82615
http://hdl.handle.net/10220/49417
ISSN: 1433-7851
DOI: https://dx.doi.org/10.1002/anie.201900092
Rights: This is the peer reviewed version of the following article: Lyu, Y., Cui, D., Huang, J., Fan, W., Miao, Y., & Pu, K. (2019). Near-infrared afterglow semiconducting nano-polycomplexes for the multiplex differentiation of cancer exosomes. Angewandte Chemie International Edition, 58(15), 4983-4987. doi:10.1002/anie.201900092, which has been published in final form at https://dx.doi.org/10.1002/anie.201900092. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: embargo_20200408
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SCBE Journal Articles

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