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|Title:||Near-infrared afterglow semiconducting nano-polycomplexes for multiplex differentiation of cancer exosomes||Authors:||Lyu, Yan
|Issue Date:||2019||Source:||Lyu, Y., Cui, D., Huang, J., Fan, W., Miao, Y., & Pu, K. (2019). Near-infrared afterglow semiconducting nano-polycomplexes for the multiplex differentiation of cancer exosomes. Angewandte Chemie International Edition, 58(15), 4983-4987. doi:10.1002/anie.201900092||Series/Report no.:||Angewandte Chemie International Edition||Abstract:||The detection of exosomes is promising for the early diagnosis of cancer. However, the development of suitable optical sensors remains challenging. We have developed the first luminescent nanosensor for the multiplex differentiation of cancer exosomes that bypasses real‐time light excitation. The sensor is composed of a near‐infrared semiconducting polyelectrolyte (ASPN) that forms a complex with a quencher‐tagged aptamer. The afterglow signal of the nanocomplex (ASPNC), being initially quenched, is turned on in the presence of aptamer‐targeted exosomes. Because detection of the afterglow takes place after the excitation, background signals are minimized, leading to an improved limit of detection that is nearly two orders of magnitude lower than that of fluorescence detection in cell culture media. Also, ASPNC can be easily tailored to detect different exosomal proteins by changing the aptamer sequence. This enables an orthogonal analysis of multiple exosome samples, potentially permitting an accurate identification of the cellular origin of exosomes for cancer diagnosis.||URI:||https://hdl.handle.net/10356/82615
|ISSN:||1433-7851||DOI:||https://dx.doi.org/10.1002/anie.201900092||Rights:||This is the peer reviewed version of the following article: Lyu, Y., Cui, D., Huang, J., Fan, W., Miao, Y., & Pu, K. (2019). Near-infrared afterglow semiconducting nano-polycomplexes for the multiplex differentiation of cancer exosomes. Angewandte Chemie International Edition, 58(15), 4983-4987. doi:10.1002/anie.201900092, which has been published in final form at https://dx.doi.org/10.1002/anie.201900092. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.||Fulltext Permission:||embargo_20200408||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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