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Title: Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis
Authors: Wu, Pei-Jung
Masouleh, Maryam Imani
Dini, Daniele
Paterson, Carl
Török, Peter
Overby, Darryl R.
Kabakova, Irina V.
Keywords: Elastic Scattering
Brillouin Microscopy
Issue Date: 2019
Source: Wu, P.-J., Masouleh, M. I., Dini, D., Paterson, C., Török, P., Overby, D. R., & Kabakova, I. V. (2019). Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis. Biomedical Optics Express, 10(5), 2457-2466. doi:10.1364/BOE.10.002457
Series/Report no.: Biomedical Optics Express
Abstract: The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early disease-related changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan (PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.
DOI: 10.1364/BOE.10.002457
Rights: © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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