Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87104
Title: A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation
Authors: Hou, Wen
Liu, Lei
Zhang, Xiaohong
Liu, Chuanfa
Keywords: Science::Biological sciences
Sequential Peptide Ligation
Thioacid Capture Ligation
Issue Date: 2018
Source: Hou, W., Liu, L., Zhang, X., & Liu, C. (2018). A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation. Royal Society Open Science, 5(6), 172455-. doi:10.1098/rsos.172455
Series/Report no.: Royal Society Open Science
Abstract: Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.
URI: https://hdl.handle.net/10356/87104
http://hdl.handle.net/10220/49869
DOI: http://dx.doi.org/10.1098/rsos.172455
Rights: © 2018 The Author(s). Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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