Please use this identifier to cite or link to this item:
|Title:||A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation||Authors:||Hou, Wen
Sequential Peptide Ligation
Thioacid Capture Ligation
|Issue Date:||2018||Source:||Hou, W., Liu, L., Zhang, X., & Liu, C. (2018). A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation. Royal Society Open Science, 5(6), 172455-. doi:10.1098/rsos.172455||Series/Report no.:||Royal Society Open Science||Abstract:||Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.||URI:||https://hdl.handle.net/10356/87104
|DOI:||http://dx.doi.org/10.1098/rsos.172455||Rights:||© 2018 The Author(s). Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.