dc.contributor.authorJames, Christina
dc.contributor.authorZhao, Tian Yun
dc.contributor.authorRahim, Anisa
dc.contributor.authorSaxena, Parul
dc.contributor.authorNazreen Abdul Muthalif
dc.contributor.authorUemura, Takeshi
dc.contributor.authorTsuneyoshi, Norihiro
dc.contributor.authorOng, Sheena
dc.contributor.authorIgarashi, Kazuei
dc.contributor.authorLim, Chin Yan
dc.contributor.authorDunn, Norris Ray
dc.contributor.authorVardy, Leah Anne
dc.identifier.citationJames, C., Zhao, T. Y., Rahim, A., Saxena, P., Nazreen Abdul Muthalif., Uemura, T., . . . Vardy, L. A. (2018). MINDY1 is a downstream target of the polyamines and promotes embryonic stem cell self‐renewal. STEM CELLS, 36(8), 1170-1178. doi:10.1002/stem.2830en_US
dc.description.abstractEmbryonic stem cells have the ability to self‐renew or differentiate and these processes are under tight control. We previously reported that the polyamine regulator AMD1 is critical for embryonic stem cell self‐renewal. The polyamines putrescine, spermidine, and spermine are essential organic cations that play a role in a wide array of cellular processes. Here, we explore the essential role of the polyamines in the promotion of self‐renewal and identify a new stem cell regulator that acts downstream of the polyamines: MINDY1. MINDY1 protein levels are high in embryonic stem cells (ESCs) and are dependent on high polyamine levels. Overexpression of MINDY1 can promote ESC self‐renewal in the absence of the usually essential cytokine Leukemia Inhibitory Factor (LIF). MINDY1 protein is prenylated and this modification is required for its ability to promote self‐renewal. We go on to show that Mindy1 RNA is targeted for repression by mir‐710 during Neural Precursor cell differentiation. Taken together, these data demonstrate that high polyamine levels are required for ESC self‐renewal and that they function, in part, through promotion of high MINDY1 levels.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent9 p.en_US
dc.relation.ispartofseriesSTEM CELLSen_US
dc.rights© 2018 The Author(s) published by Wiley Periodicals, Inc. on behalf of AlphaMed Press. This is an open access article under the terms of the Creative Commons AttributionNonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.en_US
dc.subjectEmbryonic Stem Cellsen_US
dc.subjectScience::Biological sciencesen_US
dc.titleMINDY1 is a downstream target of the polyamines and promotes embryonic stem cell self‐renewalen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.description.versionPublished versionen_US

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