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Title: Structural basis for linezolid binding site rearrangement in the Staphylococcus aureus ribosome
Authors: Tofayel Ahmed
Belousoff, Matthew J.
Eyal, Zohar
Radjainia, Mazdak
Bamert, Rebecca S.
Matzov, Donna
Bashan, Anat
Zimmerman, Ella
Mishra, Satabdi
Cameron, David
Elmlund, Hans
Peleg, Anton Y.
Bhushan, Shashi
Lithgow, Trevor
Yonath, Ada
Keywords: Staphylococcus
Antibiotic Resistance
Science::Biological sciences
Issue Date: 2017
Source: Belousoff, M. J., Eyal, Z., Radjainia, M., Tofayel Ahmed., Bamert, R. S., Matzov, D., . . . Yonath, A. (2017). Structural basis for linezolid binding site rearrangement in the Staphylococcus aureus ribosome. mBio, 8(3), e00395-17-. doi:10.1128/mBio.00395-17
Series/Report no.: mBio
Abstract: An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in the 70S ribosomes from a clinical isolate of Staphylococcus aureus. This high-resolution structural information demonstrated that a single amino acid deletion in ribosomal protein uL3 confers linezolid resistance despite being located 24 Å away from the linezolid binding pocket in the peptidyl-transferase center. The mutation induces a cascade of allosteric structural rearrangements of the rRNA that ultimately results in the alteration of the antibiotic binding site.
DOI: 10.1128/mBio.00395-17
Rights: © 2017 Belousoff et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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