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https://hdl.handle.net/10356/80710
Title: | Cell-selective pore forming antimicrobial peptides of the prodomain of human furin : a conserved aromatic/cationic sequence mapping, membrane disruption, and atomic-resolution structure and dynamics | Authors: | Sinha, Sheetal Harioudh, Munesh Kumar Dewangan, Rikeshwer P. Ng, Wun Jern Ghosh, Jimut Kanti Bhattacharjya, Surajit |
Keywords: | Pore Forming Human Furin Science::Biological sciences |
Issue Date: | 2018 | Source: | Sinha, S., Harioudh, M. K., Dewangan, R. P., Ng, W. J., Ghosh, J. K., & Bhattacharjya, S. (2018). Cell-selective pore forming antimicrobial peptides of the prodomain of human furin : a conserved aromatic/cationic sequence mapping, membrane disruption, and atomic-resolution structure and dynamics. ACS Omega, 3(11), 14650-14664. doi:10.1021/acsomega.8b01876 | Series/Report no.: | ACS Omega | Abstract: | Antimicrobial peptides are promising molecules in uprising consequences of drug-resistant bacteria. The prodomain of furin, a serine protease, expressed in all vertebrates including humans, is known to be important for physiological functions. Here, potent antimicrobial peptides were mapped by extensive analyses of overlapping peptide fragments of the prodomain of human furin. Two peptides, YR26 and YR23, were active against bacterial cells including MRSA-resistant Staphylococcus aureus and Staphylococcus epidermis 51625. Peptides were largely devoid of hemolytic and cytotoxic activity. Bacterial cell killing occurred as a result of the disruption of the permeability barrier of the lipopolysaccharide (LPS)-outer membrane and fragmentation of LPS into small micelles. Furthermore, antibacterial peptides specifically interacted with the negatively charged lipids causing membrane leakage and fusion. The YR26 peptide in sodium dodecyl sulfate micelles demonstrated a long-helix-turn-short-helix structure exhibiting restricted backbone motions. The cell-selective activity of the furin peptides and their unique mode of action on membranes have a significant potential for the development of therapeutics. | URI: | https://hdl.handle.net/10356/80710 http://hdl.handle.net/10220/50373 |
DOI: | 10.1021/acsomega.8b01876 | Schools: | School of Civil and Environmental Engineering School of Biological Sciences Interdisciplinary Graduate School (IGS) |
Organisations: | Environmental Bio-Innovation Group Advanced Environmental Biotechnology Centre |
Research Centres: | Nanyang Environment and Water Research Institute | Rights: | © 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
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acsomega.8b01876.pdf | 6.51 MB | Adobe PDF | View/Open |
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