Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition
Wang, J. X.
Cai, X. P.
Li, Chang Ming
Date of Issue2006
School of Materials Science and Engineering
We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm−2 mM−1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.
Applied physics letters
© 2006 American Institute of Physics. This paper was published in Applied Physics Letters and is made available as an electronic reprint (preprint) with permission of American Institute of Physics. The paper can be found at: [DOI: http://dx.doi.org/10.1063/1.2356307]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.