dc.contributor.authorAgarwal, Kan
dc.contributor.authorBaek, Kwanghee
dc.contributor.authorJeon, Choon Ju
dc.contributor.authorMiyamoto, Kenichi
dc.contributor.authorUeno, Akemichi
dc.contributor.authorYoon, Ho Sup
dc.date.accessioned2012-01-26T03:05:36Z
dc.date.available2012-01-26T03:05:36Z
dc.date.copyright1991en_US
dc.date.issued1991
dc.identifier.citationAgarwal, K., Baek, K., Jeon, C., Miyamoto, K., Ueno, A., & Yoon, H. (1991). Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS. Biochemistry, 30(31), 7842-7851.en_US
dc.identifier.urihttp://hdl.handle.net/10220/7480
dc.description.abstractThe eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in conjunction with the polymerase binding region for transcriptional stimulation. Interestingly, a mutant TFIIS with only the polymerase binding domain actually inhibits transcription, whereas a mutant in which the polymerase binding and zinc finger domains are separated by an octapeptide is only weakly active. The zinc finger itself has no effect on transcription, but in contrast to the wild-type protein, it binds to oligonucleotides. These findings suggest that TFIIS may interact with RNA polymerase II such that the normally masked zinc finger can specifically contact nucleotides in the transcription elongation zone at a position juxtaposed to the polymerization site.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesBiochemistryen_US
dc.rights© 1991 American Chemical Society.en_US
dc.subjectDRNTU::Science::Chemistry::Biochemistry
dc.titleStimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIISen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1021/bi00245a026


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record