Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots
Date of Issue2011
School of Chemical and Biomedical Engineering
It has been increasingly recognized that adenosine-5 ′ -triphosphate (ATP) signaling through P2 purinergic receptors has a diverse infl uence on cell functions and human health. In this contribution, a simple and non-invasive method to specifi cally label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular traffi cking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal refl ection fl uorescence microscopy.
Advanced functional materials
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