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Title: A circular ferrofluid driven microchip for rapid polymerase chain reaction
Authors: Sun, Yi
Kwok, Yien Chian
Nguyen, Nam-Trung
Keywords: DRNTU::Engineering::Mechanical engineering
Issue Date: 2007
Source: Sun, Y., Kwok, Y. C., & Nguyen, N. T. (2007). A Circular Ferrofluid Driven Microchip for Rapid Polymerase Chain Reaction. Lab on a Chip, 7(8), 1012-1017.
Series/Report no.: Lab on a chip
Abstract: In the past few years, much attention has been paid to the development of miniaturized polymerase chain reaction (PCR) devices. After a continuous flow (CF) PCR chip was introduced, several CFPCR systems employing various pumping mechanisms were reported. However, the use of pumps increases cost and imposes a high requirement on microchip bonding integrity due to the application of high pressure. Other significant limitations of CFPCR devices include the large footprint of the microchip and the fixed cycle number which is dictated by the channel layout. In this paper, we present a novel circular close-loop ferrofluid driven microchip for rapid PCR. A small ferrofluid plug, containing sub-domain magnetic particles in a liquid carrier, is driven by an external magnet along the circular microchannel, which in turn propels the PCR mixture through three temperature zones. Amplification of a 500 bp lambda DNA fragment has been demonstrated on the polymethyl methacrylate (PMMA) PCR microchip fabricated by CO2 laser ablation and bonded by a low pressure, high temperature technique. Successful PCR was achieved in less than 4 min. Effects of cycle number and cycle time on PCR products were investigated. Using a magnet as the actuator eliminates the need for expensive pumps and provides advantages of low cost, small power consumption, low requirement on bonding strength and flexible number of PCR cycles. Furthermore, the microchip has a much simpler design and smaller footprint compared to the rectangular serpentine CFPCR devices. To demonstrate its application in forensics, a 16-loci short tandem repeat (STR) sample was successfully amplified using the PCR microchip
DOI: 10.1039/B700575J
Rights: © 2007 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Lab on a Chip, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: DOI: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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