dc.contributor.authorLiew, Woei Chang
dc.contributor.authorBartfai, Richard
dc.contributor.authorLim, Zijie
dc.contributor.authorSreenivasan, Rajini
dc.contributor.authorSiegfried, Kellee R.
dc.contributor.authorOrban, Laszlo
dc.date.accessioned2013-02-20T07:14:17Z
dc.date.available2013-02-20T07:14:17Z
dc.date.copyright2012en_US
dc.date.issued2012
dc.identifier.citationLiew, W. C., Bartfai, R., Lim, Z., Sreenivasan, R., Siegfried, K. R., & Orban, L. (2012). Polygenic sex determination system in zebrafish. PLoS ONE, 7(4).en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10220/9199
dc.description.abstractDespite the popularity of zebrafish as a research model, its sex determination (SD) mechanism is still unknown. Most cytogenetic studies failed to find dimorphic sex chromosomes and no primary sex determining switch has been identified even though the assembly of zebrafish genome sequence is near to completion and a high resolution genetic map is available. Recent publications suggest that environmental factors within the natural range have minimal impact on sex ratios of zebrafish populations. The primary aim of this study is to find out more about how sex is determined in zebrafish. Methodology/Principal Findings: Using classical breeding experiments, we found that sex ratios across families were wide ranging (4.8% to 97.3% males). On the other hand, repeated single pair crossings produced broods of very similar sex ratios, indicating that parental genotypes have a role in the sex ratio of the offspring. Variation among family sex ratios was reduced after selection for breeding pairs with predominantly male or female offspring, another indication that zebrafish sex is regulated genetically. Further examinations by a PCR-based “blind assay" and array comparative genomic hybridization both failed to find universal sex-linked differences between the male and female genomes. Together with the ability to increase the sex bias of lines by selective breeding, these data suggest that zebrafish is unlikely to utilize a chromosomal sex determination (CSD) system.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesPLoS ONEen_US
dc.rights© 2012 The Authors.en_US
dc.titlePolygenic sex determination system in zebrafishen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0034397
dc.description.versionPublished versionen_US


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