Total-internal-reflection-fluorescence microscopy for the study of nanobubble dynamics
Chan, Chon U.
Date of Issue2012
School of Physical and Mathematical Sciences
Nanobubbles can be observed with optical microscopy using the total-internal-reflection-fluorescence excitation. We report on total-internal-reflection-fluorescence visualization using rhodamine 6G at 5 μM concentration which results in strongly contrasting pictures. The preferential absorption and the high spatial resolution allow us to detect nanobubbles with diameters of 230 nm and above. We resolve the nucleation dynamics during the water-ethanol-water exchange: within 4 min after exchange the bubbles nucleate and form a stable population. Additionally, we demonstrate that tracer particles near to the nanobubbles are following Brownian motion: the remaining drift flow is weaker than a few micrometers per second at a distance of 400 nm from the nanobubble’s center.
Physical review letters
© 2012 American Physical Society. This paper was published in Physical Review Letters and is made available as an electronic reprint (preprint) with permission of American Physical Society. The paper can be found at the following official DOI: [http://dx.doi.org/10.1103/PhysRevLett.109.174501]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.