Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/95674
Title: Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma
Authors: Wang, Lei
Shi, Yu
Ju, Peijun
Liu, Rui
Yeo, Siok Ping
Xia, Yinyan
Owlanj, Hamed
Feng, Zhiwei
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Wang, L., Shi, Y., Ju, P., Liu, R., Yeo, S. P., Xia, Y., et al. (2012). Silencing of Diphthamide Synthesis 3 (Dph3) Reduces Metastasis of Murine Melanoma. PLoS ONE, 7(11).
Series/Report no.: PLoS ONE
Abstract: Melanoma is the most dangerous skin cancer due to its highly metastatic potential and resistance to chemotherapy. Currently, there is no effective treatment for melanoma once it is progressed to metastatic stage. Therefore, further study to elucidate the molecular mechanism underlying the metastasis of melanoma cells is urgently required for the improvement of melanoma treatment. In the present study, we found that diphthamide synthesis 3 (Dph3) is involved in the metastasis of B16F10 murine melanoma cells by insertional mutagenesis. We demonstrated that Dph3 disruption impairs the migration of B16F10 murine melanoma cells. The requirement of Dph3 in the migration of melanoma cells was further confirmed by gene silencing with siRNA in vitro. In corresponding to this result, overexpression of Dph3 significantly promoted the migratory ability of B16F10 and B16F0 melanoma cells. Moreover, down regulation of Dph3 expression in B16F10 melanoma cells strikingly inhibits their cellular invasion and metastasis in vivo. Finally, we found that Dph3 promotes melanoma migration and invasion through the AKT signaling pathway. To conclude, our findings suggest a novel mechanism underlying the metastasis of melanoma cells which might serve as a new intervention target for the treatment of melanoma.
URI: https://hdl.handle.net/10356/95674
http://hdl.handle.net/10220/9347
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0049988
Rights: © 2012 The Authors.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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