dc.contributor.authorHew, Kelly
dc.contributor.authorDahlroth, Sue-Li
dc.contributor.authorVenkatachalam, Rajakannan
dc.contributor.authorNasertorabi, Fariborz
dc.contributor.authorLim, Bee Ting
dc.contributor.authorCornvik, Tobias Carl
dc.contributor.authorNordlund, Pär
dc.date.accessioned2013-05-23T08:14:14Z
dc.date.available2013-05-23T08:14:14Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.identifier.citationHew, K., Dahlroth, S. L., Venkatachalam, R., Nasertorabi, F., Lim, B. T., Cornvik, T. C., et al. (2013). The crystal structure of the DNA-binding domain of vIRF-1 from the oncogenic KSHV reveals a conserved fold for DNA binding and reinforces its role as a transcription factor. Nucleic acids research, 41(7), 4295-4306.en_US
dc.identifier.urihttp://hdl.handle.net/10220/9985
dc.description.abstractKaposi’s sarcoma-associated herpesvirus encodes four viral homologues to cellular interferon regulatory factors (IRFs), where the most studied is vIRF-1. Even though vIRF-1 shows sequence homology to the N-terminal DNA-binding domain (DBD) of human IRFs, a specific role for this domain in vIRF-1’s function has remained uncertain. To provide insights into the function of the vIRF-1 DBD, we have determined the crystal structure of it in complex with DNA and in its apo-form. Using a thermal stability shift assay (TSSA), we show that the vIRF-1 DBD binds DNA, whereas full-length vIRF-1 does not, suggesting a cis-acting regulatory mechanism in similarity to human IRFs. The complex structure of vIRF-1 DBD reveals interactions with the DNA backbone and the positioning of two arginines for specific recognition in the major grove. A superimposition with human IRF-3 reveals a similar positioning of the two specificity-determining arginines, and additional TSSAs indicate binding of vIRF-1 to an IRF-3 operator consensus sequence. The results from this study, therefore, provide support that vIRF-1 has evolved to bind DNA and plays a role in DNA binding in the context of transcriptional regulation and might act on some of the many operator sequences controlled by human IRF-3.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesNucleic acids researchen_US
dc.rights© 2013 The Author(s). This paper was published in Nucleic Acids Research and is made available as an electronic reprint (preprint) with permission of The Author(s). The paper can be found at the following official DOI: [http://dx.doi.org/10.1093/nar/gkt082].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en_US
dc.subjectDRNTU::Science::Biological sciences::Genetics
dc.titleThe crystal structure of the DNA-binding domain of vIRF-1 from the oncogenic KSHV reveals a conserved fold for DNA binding and reinforces its role as a transcription factoren_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gkt082
dc.description.versionPublished versionen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record