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Title: Glycophorin C (CD236R) mediates vivax malaria parasite rosetting to normocytes
Authors: Malleret, Benoit
Lau, Yee-Ling
Lee, Wenn-Chyau
Mauduit, Marjorie
Fong, Mun-Yik
Cho, Jee Sun
Suwanarusk, Rossarin
Zhang, Rou
Albrecht, Letusa
Costa, Fabio T. M.
Preiser, Peter
McGready, Rose
Renia, Laurent
Nosten, Francois
Russell, Bruce
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2014
Source: Lee, W.-C., Malleret, B., Lau, Y.-L., Mauduit, M., Fong, M.-Y., Cho, J. S., et al. (2014). Glycophorin C (CD236R) mediates vivax malaria parasite rosetting to normocytes. Blood, 123(18), e100-e109.
Series/Report no.: Blood
Abstract: Rosetting phenomenon has been linked to malaria pathogenesis. While rosetting occurs in all causes of human malaria, most data on this subject has been derived from Plasmodium falciparum. Here we investigate the function and factors affecting rosette formation in Plasmodium vivax. To achieve this, we utilised a range of novel ex vivo protocols to study fresh and cryopreserved P. vivax (n=135) and P. falciparum (n=77) isolates from Thailand. Rosetting is more common in vivax than falciparum malaria, both in terms of incidence in patient samples and percentage of infected erythrocytes forming rosettes. Rosetting to P. vivax asexual and sexual stages was evident 20 hrs post reticulocyte invasion, reaching a plateau after 30 hrs. Host ABO blood group, reticulocyte count and parasitemia were not correlated with P. vivax rosetting. Importantly, mature erythrocytes (normocytes) rather than reticulocytes preferentially form rosetting complexes, indicating this process is unlikely to directly facilitate merozoite invasion. While antibodies against host erythrocyte receptors CD235a and CD35 had no effect; Fab against the BRIC 4 region of CD236R significantly inhibited rosette formation. Rosetting assays using CD236R knock down normocytes derived from hematopoietic stem cells, further supports the role of Glycophorin C as a receptor in P. vivax rosette formation.
DOI: 10.1182/blood-2013-12-541698
Rights: © 2014 American Society of Hematology.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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