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|Title:||β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films||Authors:||Low, Yuen Kei Adarina
Ng, Kee Woei
Boey, Freddy Yin Chiang
|Keywords:||DRNTU::Engineering::Materials||Issue Date:||2013||Source:||Low, Y. K. A., Zou, X., Fang, Y., Wang, J., Lin, W., Boey, F., et al. (2014). β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films. Materials science and engineering : C, 34(1), 345-353.||Series/Report no.:||Materials science and engineering : C||Abstract:||The piezoelectric response from β-phase poly (vinylidene fluoride) (PVDF) can potentially be exploited for biomedical application. We hypothesized that α and β-phase PVDF exert direct but different influence on cellular behavior. α- and β-phase PVDF films were synthesized through solution casting and characterized with FT-IR, XRD, AFM and PFM to ensure successful fabrication of α and β-phase PVDF films. Cellular evaluation with L929 mouse fibroblasts over one-week was conducted with AlamarBlue® metabolic assay and PicoGreen® proliferation assay. Immunostaining of fibronectin investigated the extent and distribution of extracellular matrix deposition. Image saliency analysis quantified differences in cellular distribution on the PVDF films. Our results showed that β-phase PVDF films with the largest area expressing piezoelectric effect elicited highest cell metabolic activity at day 3 of culture. Increased fibronectin adsorption towards the cell-material interface was shown on β-phase PVDF films. Image saliency analysis showed that fibroblasts on β-phase PVDF films were more homogeneously distributed than on α-phase PVDF films. Taken collectively, the different molecular packing of α and β-phase PVDF resulted in differing physical properties of films, which in turn induced differences in cellular behaviors. Further analysis of how α and β-phase PVDF may evoke specific cellular behavior to suit particular application will be intriguing.||URI:||https://hdl.handle.net/10356/100215
|ISSN:||0928-4931||DOI:||http://dx.doi.org/10.1016/j.msec.2013.09.029||Rights:||© 2013 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Materials Science and Engineering C, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.msec.2013.09.029].||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
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