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Title: Disruption of putrescine biosynthesis in shewanella oneidensis enhances biofilm cohesiveness and performance in Cr(VI) immobilization
Authors: Ding, Yuanzhao
Peng, Ni
Du, Yonghua
Ji, Lianghui
Cao, Bin
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2014
Source: Ding, Y., Peng, N., Du, Y., Ji, L., & Cao, B. (2014). Disruption of putrescine biosynthesis in shewanella oneidensis enhances biofilm cohesiveness and performance in Cr(VI) immobilization. Applied and environmental microbiology, 80(4), 1498-1506.
Series/Report no.: Applied and environmental microbiology
Abstract: Although biofilm-based bioprocesses have been increasingly used in various applications, the long-term robust and efficient biofilm performance remains one of the main bottlenecks. In this study, we demonstrated that biofilm cohesiveness and performance of Shewanella oneidensis can be enhanced through disrupting putrescine biosynthesis. Through random transposon mutagenesis library screening, one hyperadherent mutant strain, CP2-1-S1, exhibiting an enhanced capability in biofilm formation, was obtained. Comparative analysis of the performance of biofilms formed by S. oneidensis MR-1 wild type (WT) and CP2-1-S1 in removing dichromate (Cr2O72−), i.e., Cr(VI), from the aqueous phase showed that, compared with the WT biofilms, CP2-1-S1 biofilms displayed a substantially lower rate of cell detachment upon exposure to Cr(VI), suggesting a higher cohesiveness of the mutant biofilms. In addition, the amount of Cr(III) immobilized by CP2-1-S1 biofilms was much larger, indicating an enhanced performance in Cr(VI) bioremediation. We further showed that speF, a putrescine biosynthesis gene, was disrupted in CP2-1-S1 and that the biofilm phenotypes could be restored by both genetic and chemical complementations. Our results also demonstrated an important role of putrescine in mediating matrix disassembly in S. oneidensis biofilms.
ISSN: 0099-2240
DOI: 10.1128/AEM.03461-13
Rights: © 2014 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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