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https://hdl.handle.net/10356/100610
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sze, Siu Kwan | en |
dc.contributor.author | Hao, Piliang | en |
dc.contributor.author | Ren, Yan | en |
dc.contributor.author | Pasterkamp, Gerard | en |
dc.contributor.author | Moll, Frans L. | en |
dc.contributor.author | de Kleijn, Dominique P. V. | en |
dc.date.accessioned | 2014-10-29T02:48:30Z | en |
dc.date.accessioned | 2019-12-06T20:25:20Z | - |
dc.date.available | 2014-10-29T02:48:30Z | en |
dc.date.available | 2019-12-06T20:25:20Z | - |
dc.date.copyright | 2014 | en |
dc.date.issued | 2014 | en |
dc.identifier.citation | Hao, P., Ren, Y., Pasterkamp, G., Moll, F. L., de Kleijn, D. P. V., & Sze, S. K. (2014). Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS. PROTEOMICS - clinical applications, 8(7-8), 631-635. | en |
dc.identifier.issn | 1862-8346 | en |
dc.identifier.uri | https://hdl.handle.net/10356/100610 | - |
dc.description.abstract | Purpose: To increase the proteome coverage of human atherosclerotic plaques and identify low-abundance proteins that may have important functions during the development and progression of atherosclerosis. Experimental design: Thirty-eight human carotid atherosclerotic plaques were pooled into two samples and analyzed in triplicate using offline multidimensional LC-MS/MS. The collected fractions of trypsin-digested peptides from Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) were analyzed by LC-MS/MS on a Q Exactive (Thermo Fisher, MA, USA). Results: A total of 4702 proteins were identified from atherosclerotic plaques at a false discovery rate (FDR) of 1%, of which 3846 were identified with at least 2 unique peptides. Many pathways related to the development and progression of atherosclerosis were identified, such as atherosclerosis signaling, toll receptor signaling pathway and inhibition of matrix metalloproteases. Many low-abundance proteins with important functions in atherosclerosis that were previously unidentifiable using mass spectrometry based proteomics methods, such as TGF-β, interleukins and other growth factors, were identified confidently from plaques. Conclusions and clinical relevance: This study has substantially increased the coverage of the atherosclerotic plaque proteome which represents a leap forward in understanding of plaque composition, development and progression. The identification of many low-abundance proteins may also facilitate biomarker discovery. | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | PROTEOMICS - clinical applications | en |
dc.rights | © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | en |
dc.subject | DRNTU::Science::Biological sciences::Human anatomy and physiology | en |
dc.title | Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS | en |
dc.type | Journal Article | en |
dc.contributor.school | School of Biological Sciences | en |
dc.contributor.organization | Singapore Centre for Environmental Life Sciences Engineering | en |
dc.identifier.doi | 10.1002/prca.201400007 | en |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | SBS Journal Articles SCELSE Journal Articles |
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