Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/100940
Title: A whole cell pathway screen reveals seven novel chemosensitizers to combat chloroquine resistant malaria
Authors: Ch'ng, Jun-Hong
Mok, Sachel
Bozdech, Zbynek
Lear, Martin James
Boudhar, Aicha
Russell, Bruce
Nosten, Francois
Tan, Kevin Shyong-Wei
Keywords: DRNTU::Science::Biological sciences::Biochemistry
Issue Date: 2013
Source: Ch'ng, J.-H., Mok, S., Bozdech, Z., Lear, M. J., Boudhar, A., Russell, B., Nosten, F., & Tan, K. S.-W. (2013). A whole cell pathway screen reveals seven novel chemosensitizers to combat chloroquine resistant malaria. Scientific reports, 3: 1734.
Series/Report no.: Scientific reports
Abstract: Due to the widespread prevalence of resistant parasites, chloroquine (CQ) was removed from front-line antimalarial chemotherapy in the 1990s despite its initial promise of disease eradication. Since then, resistance-conferring mutations have been identified in transporters such as the PfCRT, that allow for the efflux of CQ from its primary site of action, the parasite digestive vacuole. Chemosensitizing/ chemoreversing compounds interfere with the function of these transporters thereby sensitizing parasites to CQ once again. However, compounds identified thus far have disappointing in vivo efficacy and screening for alternative candidates is required to revive this strategy. In this study, we propose a simple and direct means to rapidly screen for such compounds using a fluorescent-tagged CQ molecule. When this screen was applied to a small library, seven novel chemosensitizers (octoclothepin, methiothepin, metergoline, loperamide, chlorprothixene, L-703,606 and mibefradil) were quickly elucidated, including two which showed greater potency than the classical chemosensitizers verapamil and desipramine.
URI: https://hdl.handle.net/10356/100940
http://hdl.handle.net/10220/16522
ISSN: 2045-2322
DOI: 10.1038/srep01734
Rights: © 2013 The Author(s).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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