Please use this identifier to cite or link to this item:
|Title:||Selectivity of stop codon recognition in translation termination is modulated by multiple conformations of GTS loop in eRF1||Authors:||Li, Yan
Wong, Leo E.
|Keywords:||DRNTU::Science::Biological sciences||Issue Date:||2012||Source:||Wong, L. E., Li, Y., Pillay, S., Frolova, L., & Pervushin, K. (2012). Selectivity of stop codon recognition in translation termination is modulated by multiple conformations of GTS loop in eRF1. Nucleic acids research, 40(12), 5751-5765.||Series/Report no.:||Nucleic acids research||Abstract:||Translation termination in eukaryotes is catalyzed by two release factors eRF1 and eRF3 in a cooperative manner. The precise mechanism of stop codon discrimination by eRF1 remains obscure, hindering drug development targeting aberrations at translation termination. By solving the solution structures of the wild-type N-domain of human eRF1 exhibited omnipotent specificity, i.e. recognition of all three stop codons, and its unipotent mutant with UGA-only specificity, we found the conserved GTS loop adopting alternate conformations. We propose that structural variability in the GTS loop may underline the switching between omnipotency and unipotency of eRF1, implying the direct access of the GTS loop to the stop codon. To explore such feasibility, we positioned N-domain in a pre-termination ribosomal complex using the binding interface between N-domain and model RNA oligonucleotides mimicking Helix 44 of 18S rRNA. NMR analysis revealed that those duplex RNA containing 2-nt internal loops interact specifically with helix α1 of N-domain, and displace C-domain from a non-covalent complex of N-domain and C-domain, suggesting domain rearrangement in eRF1 that accompanies N-domain accommodation into the ribosomal A site.||URI:||https://hdl.handle.net/10356/101527
|DOI:||10.1093/nar/gks192||Rights:||© 2012 The Authors. This paper was published in Nucleic Acids Research and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1093/nar/gks192]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
Files in This Item:
|Selectivity of stop codon recognition in translation termination is modulated by multiple conformations of GTS loop in eRF1.pdf||6.26 MB||Adobe PDF|
Updated on Feb 12, 2022
Updated on Feb 11, 2022
Page view(s) 20537
Updated on Jun 27, 2022
Updated on Jun 27, 2022
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.