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|Title:||Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes||Authors:||De Hoog, Hans-Peter M.
Lin JieRong, Esther M.
Décaillot, Fabien M.
|Keywords:||DRNTU::Engineering::Materials::Biomaterials||Issue Date:||2014||Source:||De Hoog, H.-P. M., Lin JieRong, E. M., Banerjee, S., Décaillot, F. M., & Nallani, M. (2014). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS One, 9(10), e110847-.||Series/Report no.:||PLoS One||Abstract:||G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4.||URI:||https://hdl.handle.net/10356/101602
|ISSN:||1932-6203||DOI:||10.1371/journal.pone.0110847||Rights:||© 2014 de Hoog et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
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