Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/101602
Title: Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes
Authors: De Hoog, Hans-Peter M.
Lin JieRong, Esther M.
Banerjee, Sourabh
Décaillot, Fabien M.
Nallani, Madhavan
Keywords: DRNTU::Engineering::Materials::Biomaterials
Issue Date: 2014
Source: De Hoog, H.-P. M., Lin JieRong, E. M., Banerjee, S., Décaillot, F. M., & Nallani, M. (2014). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS One, 9(10), e110847-.
Series/Report no.: PLoS One
Abstract: G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4.
URI: https://hdl.handle.net/10356/101602
http://hdl.handle.net/10220/24190
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0110847
Rights: © 2014 de Hoog et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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