Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/101602
Title: | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes | Authors: | De Hoog, Hans-Peter M. Lin JieRong, Esther M. Banerjee, Sourabh Décaillot, Fabien M. Nallani, Madhavan |
Keywords: | DRNTU::Engineering::Materials::Biomaterials | Issue Date: | 2014 | Source: | De Hoog, H.-P. M., Lin JieRong, E. M., Banerjee, S., Décaillot, F. M., & Nallani, M. (2014). Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS One, 9(10), e110847-. | Series/Report no.: | PLoS One | Abstract: | G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4. | URI: | https://hdl.handle.net/10356/101602 http://hdl.handle.net/10220/24190 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0110847 | Rights: | © 2014 de Hoog et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | MSE Journal Articles |
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Conformational Antibody Binding to a Native, Cell-Free Expressed GPCR in Block Copolymer Membranes.pdf | 571.85 kB | Adobe PDF | ![]() View/Open |
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