Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/102550
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBonanni, Alessandraen
dc.contributor.authorChua, Chun Kiangen
dc.contributor.authorPumera, Martinen
dc.date.accessioned2014-04-03T08:00:56Zen
dc.date.accessioned2019-12-06T20:56:50Z-
dc.date.available2014-04-03T08:00:56Zen
dc.date.available2019-12-06T20:56:50Z-
dc.date.copyright2014en
dc.date.issued2014en
dc.identifier.citationBonanni, A., Chua, C. K., & Pumera, M. (2014). Rational design of carboxyl groups perpendicularly attached to a graphene sheet : a platform for enhanced biosensing applications. Chemistry - A European Journal, 20(1), 217-222.en
dc.identifier.issn0947-6539en
dc.identifier.urihttps://hdl.handle.net/10356/102550-
dc.description.abstractGraphene oxide (GO)-based materials offer great potential for biofunctionalization with applications ranging from biosensing to drug delivery. Such biofunctionalization utilizes specific functional groups, typically a carboxyl moiety, as anchoring points for biomolecule. However, due to the fact that the exact chemical structure of GO is still largely unknown and poorly defined (it was postulated to consist of various oxygen-containing groups, such as epoxy, hydroxyl, carboxyl, carbonyl, and peroxy in varying ratios), it is challenging to fabricate highly biofunctionalized GO surfaces. The predominant anchoring sites (i.e., carboxyl groups) are mainly present as terminal groups on the edges of GO sheets and thus account for only a fraction of the oxygen-containing groups on GO. Herein, we suggest a direct solution to the long-standing problem of limited abundance of carboxyl groups on GO; GO was first reduced to graphene and consequently modified with only carboxyl groups grafted perpendicularly to its surface by a rational synthesis using free-radical addition of isobutyronitrile with subsequent hydrolysis. Such grafted graphene oxide can contain a high amount of carboxyl groups for consequent biofunctionalization, at which the extent of grafting is limited only by the number of carbon atoms in the graphene plane; in contrast, the abundance of carboxyl groups on “classical” GO is limited by the amount of terminal carbon atoms. Such a graphene platform embedded with perpendicularly grafted carboxyl groups was characterized in detail by X-ray photoelectron spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy, and its application was exemplified with single-nucleotide polymorphism detection. It was found that the removal of oxygen functionalities after the chemical reduction enhanced the electron-transfer rate of the graphene. More importantly, the introduction of carboxyl groups promoted a more efficient immobilization of DNA probes on the electrode surface and improved the performance of graphene as a biosensor in comparison to GO. The proposed material can be used as a universal platform for biomolecule immobilization to facilitate rapid and sensitive detection of DNA or proteins for point-of-care investigations. Such reactive carboxyl groups grafted perpendicularly on GO holds promise for a highly efficient tailored biofunctionalization for applications in biosensing or drug delivery.en
dc.language.isoenen
dc.relation.ispartofseriesChemistry - a European journalen
dc.rights© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.subjectDRNTU::Science::Chemistryen
dc.titleRational design of carboxyl groups perpendicularly attached to a graphene sheet : a platform for enhanced biosensing applicationsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen
dc.identifier.doi10.1002/chem.201303582en
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:SPMS Journal Articles

SCOPUSTM   
Citations 10

34
Updated on Sep 7, 2020

PublonsTM
Citations 5

35
Updated on Mar 6, 2021

Page view(s) 20

425
Updated on Apr 22, 2021

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.