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https://hdl.handle.net/10356/102791| Title: | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments | Authors: | Yildiz, Umit Hakan De Hoog, Hans-Peter M. Fu, Zhikang Tomczak, Nikodem Parikh, Atul N. Nallani, Madhavan Liedberg, Bo |
Keywords: | DRNTU::Engineering::Materials | Issue Date: | 2013 | Source: | Yildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447. | Series/Report no.: | Small | Abstract: | Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase. | URI: | https://hdl.handle.net/10356/102791 http://hdl.handle.net/10220/19139 |
ISSN: | 1613-6810 | DOI: | 10.1002/smll.201300060 | Schools: | School of Materials Science & Engineering | Rights: | © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | Fulltext Permission: | none | Fulltext Availability: | No Fulltext |
| Appears in Collections: | MSE Journal Articles |
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