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Title: Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments
Authors: Yildiz, Umit Hakan
De Hoog, Hans-Peter M.
Fu, Zhikang
Tomczak, Nikodem
Parikh, Atul N.
Nallani, Madhavan
Liedberg, Bo
Keywords: DRNTU::Engineering::Materials
Issue Date: 2013
Source: Yildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447.
Series/Report no.: Small
Abstract: Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
ISSN: 1613-6810
DOI: 10.1002/smll.201300060
Rights: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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