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|Title:||Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments||Authors:||Yildiz, Umit Hakan
De Hoog, Hans-Peter M.
Parikh, Atul N.
|Keywords:||DRNTU::Engineering::Materials||Issue Date:||2013||Source:||Yildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447.||Series/Report no.:||Small||Abstract:||Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.||URI:||https://hdl.handle.net/10356/102791
|ISSN:||1613-6810||DOI:||10.1002/smll.201300060||Rights:||© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||MSE Journal Articles|
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