Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103049
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dc.contributor.authorKline, Kimberly A.en
dc.contributor.authorKorte-Berwanger, Miriamen
dc.contributor.authorSakinc, Türkanen
dc.contributor.authorNielsen, Hailyn V.en
dc.contributor.authorHultgren, Scotten
dc.contributor.authorGatermann, Sören G.en
dc.contributor.editorCamilli, A.en
dc.date.accessioned2015-06-04T08:44:26Zen
dc.date.accessioned2019-12-06T21:04:27Z-
dc.date.available2015-06-04T08:44:26Zen
dc.date.available2019-12-06T21:04:27Z-
dc.date.copyright2013en
dc.date.issued2013en
dc.identifier.citationKorte-Berwanger, M., Sakinc, T., Kline, K., Nielsen, H. V., Hultgren, S., & Gatermann, S. G. (2013). Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence. Infection and immunity, 81(12), 4525-4533.en
dc.identifier.urihttps://hdl.handle.net/10356/103049-
dc.description.abstractStaphylococcus saprophyticus is the only species of Staphylococcus that is typically uropathogenic and possesses a gene coding for a D-serine-deaminase (DsdA). As D-serine is prevalent in urine and toxic or bacteriostatic to many bacteria, it is not surprising that the D-serine-deaminase gene is found in the genome of uropathogens. It has been suggested that D-serine-deaminase or the ability to respond to or to metabolize D-serine is important for virulence. For uropathogenic Escherichia coli (UPEC), a high intracellular D-serine concentration affects expression of virulence factors. S. saprophyticus is able to grow in the presence of high D-serine concentrations; however, its D-serine metabolism has not been described. The activity of the D-serine-deaminase was verified by analyzing the formation of pyruvate from D-serine in different strains with and without D-serine-deaminase. Cocultivation experiments were performed to show that D-serine-deaminase confers a growth advantage to S. saprophyticus in the presence of D-serine. Furthermore, in vivo coinfection experiments showed a disadvantage for the ΔdsdA mutant during urinary tract infection. Expression analysis of known virulence factors by reverse transcription-quantitative PCR (RT-qPCR) showed that the surface-associated lipase Ssp is upregulated in the presence of D-serine. In addition, we show that S. saprophyticus is able to use D-serine as the sole carbon source, but interestingly, D-serine had a negative effect on growth when glucose was also present. Taken together, D-serine metabolism is associated with virulence in S. saprophyticus, as at least one known virulence factor is upregulated in the presence of D-serine and a ΔdsdA mutant was attenuated in virulence murine model of urinary tract infection.en
dc.format.extent9 p.en
dc.language.isoenen
dc.relation.ispartofseriesInfection and immunityen
dc.rights© 2013 American Society for Microbiology (ASM). This paper was published in Infection and Immunity and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/IAI.00599-13]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Immunologyen
dc.titleSignificance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulenceen
dc.typeJournal Articleen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.identifier.doi10.1128/IAI.00599-13en
dc.description.versionPublished versionen
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