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|Title:||Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion||Authors:||Ilegems, E.
van Krieken, P. P.
Edlund, P. K.
Berggren, Per-Olof Yngve
|Issue Date:||2015||Source:||Ilegems, E., van Krieken, P. P., Edlund, P. K., Dicker, A., Alanentalo, T., Eriksson, M., et al. (2015). Light scattering as an intrinsic indicator for pancreatic islet cell mass and secretion. Scientific Reports, 5, 10740-.||Series/Report no.:||Scientific Reports||Abstract:||The pancreatic islet of Langerhans is composed of endocrine cells producing and releasing hormones from secretory granules in response to various stimuli for maintenance of blood glucose homeostasis. In order to adapt to a variation in functional demands, these islets are capable of modulating their hormone secretion by increasing the number of endocrine cells as well as the functional response of individual cells. A failure in adaptive mechanisms will lead to inadequate blood glucose regulation and thereby to the development of diabetes. It is therefore necessary to develop tools for the assessment of both pancreatic islet mass and function, with the aim of understanding cellular regulatory mechanisms and factors guiding islet plasticity. Although most of the existing techniques rely on the use of artificial indicators, we present an imaging methodology based on intrinsic optical properties originating from mature insulin secretory granules within endocrine cells that reveals both pancreatic islet mass and function. We demonstrate the advantage of using this imaging strategy by monitoring in vivo scattering signal from pancreatic islets engrafted into the anterior chamber of the mouse eye, and how this versatile and noninvasive methodology permits the characterization of islet morphology and plasticity as well as hormone secretory status.||URI:||https://hdl.handle.net/10356/103056
|ISSN:||2045-2322||DOI:||10.1038/srep10740||Rights:||This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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