Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103160
Title: Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA
Authors: Wang, Zhijuan
Zhang, Juan
Zhu, Changfeng
Wu, Shixin
Mandler, Daniel
Marks, Robert S.
Zhang, Hua
Keywords: DRNTU::Engineering::Materials::Biomaterials
Issue Date: 2014
Source: Wang, Z., Zhang, J., Zhu, C., Wu, S., Mandler, D., Marks, R. S., et al. (2014). Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA. Nanoscale, 6(6), 3110-3115.
Series/Report no.: Nanoscale
Abstract: A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling molecule (MB-T10-SH), forming DNA–AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA–Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA–Au conjugate enables the detection of target DNA in the linear range of 10−13 to 10−8 M with the detection limit as low as 50 fM.
URI: https://hdl.handle.net/10356/103160
http://hdl.handle.net/10220/24464
ISSN: 2040-3364
DOI: 10.1039/c3nr05625b
Rights: © 2014 The Royal Society of Chemistry.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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