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|Title:||Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA||Authors:||Wang, Zhijuan
Marks, Robert S.
|Keywords:||DRNTU::Engineering::Materials::Biomaterials||Issue Date:||2014||Source:||Wang, Z., Zhang, J., Zhu, C., Wu, S., Mandler, D., Marks, R. S., et al. (2014). Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA. Nanoscale, 6(6), 3110-3115.||Series/Report no.:||Nanoscale||Abstract:||A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling molecule (MB-T10-SH), forming DNA–AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA–Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA–Au conjugate enables the detection of target DNA in the linear range of 10−13 to 10−8 M with the detection limit as low as 50 fM.||URI:||https://hdl.handle.net/10356/103160
|ISSN:||2040-3364||DOI:||10.1039/c3nr05625b||Rights:||© 2014 The Royal Society of Chemistry.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||MSE Journal Articles|
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