Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103359
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKhaw, Swea-Lingen
dc.contributor.authorKoh, Cheng-Geeen
dc.contributor.authorLim, Bingen
dc.contributor.authorChua, Min-Wenen
dc.contributor.authorNg, Shyh-Changen
dc.date.accessioned2015-09-23T06:10:03Zen
dc.date.accessioned2019-12-06T21:10:50Z-
dc.date.available2015-09-23T06:10:03Zen
dc.date.available2019-12-06T21:10:50Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationKhaw, S.-L., Chua, M.-W., Koh, C.-G., Lim, B., & Ng, S.-C. (2015). Oocyte Factors Suppress Mitochondrial Polynucleotide Phosphorylase to Remodel the Metabolome and Enhance Reprogramming. Cell Reports, 12(7), 1080-1088.en
dc.identifier.issn2211-1247en
dc.identifier.urihttps://hdl.handle.net/10356/103359-
dc.description.abstractOocyte factors not only drive somatic cell nuclear transfer reprogramming but also augment the efficiency and quality of induced pluripotent stem cell (iPSC) reprogramming. Here, we show that the oocyte-enriched factors Tcl1 and Tcl1b1 significantly enhance reprogramming efficiency. Clonal analysis of pluripotency biomarkers further show that the Tcl1 oocyte factors improve the quality of reprogramming. Mechanistically, we find that the enhancement effect of Tcl1b1 depends on Akt, one of its putative targets. In contrast, Tcl1 suppresses the mitochondrial polynucleotide phosphorylase (PnPase) to promote reprogramming. Knockdown of PnPase rescues the inhibitory effect from Tcl1 knockdown during reprogramming, whereas PnPase overexpression abrogates the enhancement from Tcl1 overexpression. We further demonstrate that Tcl1 suppresses PnPase’s mitochondrial localization to inhibit mitochondrial biogenesis and oxidation phosphorylation, thus remodeling the metabolome. Hence, we identified the Tcl1-PnPase pathway as a critical mitochondrial switch during reprogrammingen
dc.language.isoenen
dc.relation.ispartofseriesCell Reportsen
dc.rights© 2015 The Authors. This paper was published in Cell Reports and is made available as an electronic reprint (preprint) with permission of The Authors. The published version is available at: [http://dx.doi.org/10.1016/j.celrep.2015.07.032]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.titleOocyte Factors Suppress Mitochondrial Polynucleotide Phosphorylase to Remodel the Metabolome and Enhance Reprogrammingen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1016/j.celrep.2015.07.032en
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SBS Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.