Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103360
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dc.contributor.authorGuo, Hui-Chenen
dc.contributor.authorJin, Yeen
dc.contributor.authorHan, Shi-Chongen
dc.contributor.authorSun, Shi-Qien
dc.contributor.authorWei, Yan-Quanen
dc.contributor.authorLiu, Xian-Jien
dc.contributor.authorFeng, Xiaen
dc.contributor.authorLiu, Ding Xiangen
dc.contributor.authorLiu, Xiang-Taoen
dc.contributor.editorJin, Dong-Yanen
dc.date.accessioned2015-09-23T04:11:48Zen
dc.date.accessioned2019-12-06T21:10:52Z-
dc.date.available2015-09-23T04:11:48Zen
dc.date.available2019-12-06T21:10:52Z-
dc.date.copyright2015en
dc.date.issued2015en
dc.identifier.citationGuo, H.-C., Jin, Y., Han, S.-C., Sun, S.-Q., Wei, Y.-Q., Liu, X.-J., et al. (2015). Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. PLOS ONE, 10(7), e0132384-.en
dc.identifier.issn1932-6203en
dc.identifier.urihttps://hdl.handle.net/10356/103360-
dc.description.abstractStable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified overall 2,141 proteins, 153 of which showed significant alteration in the expression level 6 h post FMDV infection (57 up-regulated and 96 down-regulated). Among these proteins, six cellular proteins, including three down-regulated (VPS28, PKR, EVI5) and three up-regulated (LYPLA1, SEC62 and DARs), were selected according to the significance of the changes and/or the relationship with PKR. The expression level and pattern of the selected proteins were validated by immunoblotting and confocal microscopy. Furthermore, the functions of these cellular proteins were assessed by small interfering RNA-mediated depletion, and their functional importance in the replication of FMDV was demonstrated by western blot, reverse transcript PCR (RT-PCR) and 50% Tissue Culture Infective Dose (TCID50). The results suggest that FMDV infection may have effects on the expression of specific cellular proteins to create more favorable conditions for FMDV infection. This study provides novel data that can be utilized to understand the interactions between FMDV and the host cell.en
dc.language.isoenen
dc.relation.ispartofseriesPLOS ONEen
dc.rights© 2015 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleQuantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1en
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1371/journal.pone.0132384en
dc.description.versionPublished versionen
item.grantfulltextopen-
item.fulltextWith Fulltext-
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