Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/103360
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Guo, Hui-Chen | en |
dc.contributor.author | Jin, Ye | en |
dc.contributor.author | Han, Shi-Chong | en |
dc.contributor.author | Sun, Shi-Qi | en |
dc.contributor.author | Wei, Yan-Quan | en |
dc.contributor.author | Liu, Xian-Ji | en |
dc.contributor.author | Feng, Xia | en |
dc.contributor.author | Liu, Ding Xiang | en |
dc.contributor.author | Liu, Xiang-Tao | en |
dc.contributor.editor | Jin, Dong-Yan | en |
dc.date.accessioned | 2015-09-23T04:11:48Z | en |
dc.date.accessioned | 2019-12-06T21:10:52Z | - |
dc.date.available | 2015-09-23T04:11:48Z | en |
dc.date.available | 2019-12-06T21:10:52Z | - |
dc.date.copyright | 2015 | en |
dc.date.issued | 2015 | en |
dc.identifier.citation | Guo, H.-C., Jin, Y., Han, S.-C., Sun, S.-Q., Wei, Y.-Q., Liu, X.-J., et al. (2015). Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. PLOS ONE, 10(7), e0132384-. | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | https://hdl.handle.net/10356/103360 | - |
dc.description.abstract | Stable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified overall 2,141 proteins, 153 of which showed significant alteration in the expression level 6 h post FMDV infection (57 up-regulated and 96 down-regulated). Among these proteins, six cellular proteins, including three down-regulated (VPS28, PKR, EVI5) and three up-regulated (LYPLA1, SEC62 and DARs), were selected according to the significance of the changes and/or the relationship with PKR. The expression level and pattern of the selected proteins were validated by immunoblotting and confocal microscopy. Furthermore, the functions of these cellular proteins were assessed by small interfering RNA-mediated depletion, and their functional importance in the replication of FMDV was demonstrated by western blot, reverse transcript PCR (RT-PCR) and 50% Tissue Culture Infective Dose (TCID50). The results suggest that FMDV infection may have effects on the expression of specific cellular proteins to create more favorable conditions for FMDV infection. This study provides novel data that can be utilized to understand the interactions between FMDV and the host cell. | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | PLOS ONE | en |
dc.rights | © 2015 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.title | Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1 | en |
dc.type | Journal Article | en |
dc.contributor.school | School of Biological Sciences | en |
dc.identifier.doi | 10.1371/journal.pone.0132384 | en |
dc.description.version | Published version | en |
dc.identifier.pmid | 26161868 | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
Appears in Collections: | SBS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1.pdf | 9.01 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
20
11
Updated on Mar 26, 2024
Web of ScienceTM
Citations
20
14
Updated on Oct 26, 2023
Page view(s) 50
419
Updated on Mar 28, 2024
Download(s) 10
409
Updated on Mar 28, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.