Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103360
Title: Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1
Authors: Guo, Hui-Chen
Jin, Ye
Han, Shi-Chong
Sun, Shi-Qi
Wei, Yan-Quan
Liu, Xian-Ji
Feng, Xia
Liu, Ding Xiang
Liu, Xiang-Tao
Issue Date: 2015
Source: Guo, H.-C., Jin, Y., Han, S.-C., Sun, S.-Q., Wei, Y.-Q., Liu, X.-J., et al. (2015). Quantitative Proteomic Analysis of BHK-21 Cells Infected with Foot-and-Mouth Disease Virus Serotype Asia 1. PLOS ONE, 10(7), e0132384-.
Series/Report no.: PLOS ONE
Abstract: Stable isotope labeling with amino acids in cell culture (SILAC) was used to quantitatively study the host cell gene expression profile, in order to achieve an unbiased overview of the protein expression changes in BHK-21 cells infected with FMDV serotype Asia 1. The SILAC-based approach identified overall 2,141 proteins, 153 of which showed significant alteration in the expression level 6 h post FMDV infection (57 up-regulated and 96 down-regulated). Among these proteins, six cellular proteins, including three down-regulated (VPS28, PKR, EVI5) and three up-regulated (LYPLA1, SEC62 and DARs), were selected according to the significance of the changes and/or the relationship with PKR. The expression level and pattern of the selected proteins were validated by immunoblotting and confocal microscopy. Furthermore, the functions of these cellular proteins were assessed by small interfering RNA-mediated depletion, and their functional importance in the replication of FMDV was demonstrated by western blot, reverse transcript PCR (RT-PCR) and 50% Tissue Culture Infective Dose (TCID50). The results suggest that FMDV infection may have effects on the expression of specific cellular proteins to create more favorable conditions for FMDV infection. This study provides novel data that can be utilized to understand the interactions between FMDV and the host cell.
URI: https://hdl.handle.net/10356/103360
http://hdl.handle.net/10220/38736
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0132384
Rights: © 2015 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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