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dc.contributor.authorZhu, Detuen
dc.contributor.authorZhao, Zuxianglanen
dc.contributor.authorCui, Guimeien
dc.contributor.authorChang, Shiehongen
dc.contributor.authorHu, Linglingen
dc.contributor.authorSee, Yi Xiangen
dc.contributor.authorLim, Michelle Gek Liangen
dc.contributor.authorGuo, Dajiangen
dc.contributor.authorChen, Xinen
dc.contributor.authorRobson, Paulen
dc.contributor.authorLuo, Yumeien
dc.contributor.authorCheung, Edwinen
dc.identifier.citationZhu, D., Zhao, Z., Cui, G., Chang, S., Hu, L., See, Y. X., . . . Cheung, E. (2018). Single-cell transcriptome analysis reveals estrogen signaling coordinately augments one-carbon, polyamine, and purine synthesis in breast cancer. Cell Reports, 25(8), 2285-2298. doi:10.1016/j.celrep.2018.10.093en
dc.description.abstractEstrogen drives breast cancer (BCa) progression by directly activating estrogen receptor α (ERα). However, because of the stochastic nature of gene transcription, it is important to study the estrogen signaling pathway at the single-cell level to fully understand how ERα regulates transcription. Here, we performed single-cell transcriptome analysis on ERα-positive BCa cells following 17β-estradiol stimulation and reconstructed the dynamic estrogen-responsive transcriptional network from discrete time points into a pseudotemporal continuum. Notably, differentially expressed genes show an estrogen-stimulated metabolic switch that favors biosynthesis but reduces estrogen degradation. Moreover, folate-mediated one-carbon metabolism is reprogrammed through the mitochondrial folate pathway and polyamine and purine synthesis are upregulated coordinately. Finally, we show AZIN1 and PPAT are direct ERα targets that are essential for BCa cell survival and growth. In summary, our study highlights the dynamic transcriptional heterogeneity in ERα-positive BCa cells upon estrogen stimulation and uncovers a mechanism of estrogen-mediated metabolic switch.en
dc.format.extent33 p.en
dc.relation.ispartofseriesCell Reportsen
dc.rights© 2018 The Authors. (Published by Elsevier). This is an open access article under the CC BY-NC-ND license (
dc.subjectBreast Canceren
dc.subjectSingle-Cell Transcriptomeen
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleSingle-cell transcriptome analysis reveals estrogen signaling coordinately augments one-carbon, polyamine, and purine synthesis in breast canceren
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionPublished versionen
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