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Title: A microfluidic co-culture system to monitor tumor-stromal interactions on a chip
Authors: Menon, Nishanth V.
Chuah, Yon Jin
Cao, Bin
Lim, Mayasari
Kang, Yuejun
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2014
Source: Menon, N. V., Chuah, Y. J., Cao, B., Lim, M., & Kang, Y. (2014). A microfluidic co-culture system to monitor tumor-stromal interactions on a chip. Biomicrofluidics, 8(6), 064118-.
Series/Report no.: Biomicrofluidics
Abstract: The living cells are arranged in a complex natural environment wherein they interact with extracellular matrix and other neighboring cells. Cell-cell interactions, especially those between distinct phenotypes, have attracted particular interest due to the significant physiological relevance they can reveal for both fundamental and applied biomedical research. To study cell-cell interactions, it is necessary to develop co-culture systems, where different cell types can be cultured within the same confined space. Although the current advancement in lab-on-a-chip technology has allowed the creation of in vitro models to mimic the complexity of in vivo environment, it is still rather challenging to create such co-culture systems for easy control of different colonies of cells. In this paper, we have demonstrated a straightforward method for the development of an on-chip co-culture system. It involves a series of steps to selectively change the surface property for discriminative cell seeding and to induce cellular interaction in a co-culture region. Bone marrow stromal cells (HS5) and a liver tumor cell line (HuH7) have been used to demonstrate this co-culture model. The cell migration and cellular interaction have been analyzed using microscopy and biochemical assays. This co-culture system could be used as a disease model to obtain biological insight of pathological progression, as well as a tool to evaluate the efficacy of different drugs for pharmaceutical studies.
ISSN: 1932-1058
DOI: 10.1063/1.4903762
Rights: © 2014 AIP Publishing LLC. This paper was published in Biomicrofluidics and is made available as an electronic reprint (preprint) with permission of AIP Publishing LLC. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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