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dc.contributor.authorPandey, Kishoren
dc.contributor.authorPandey, Basu Deven
dc.contributor.authorMallik, Arun Kumaren
dc.contributor.authorAcharya, Jyotien
dc.contributor.authorKato, Kentaroen
dc.contributor.authorKaneko, Osamuen
dc.contributor.authorFerreira, Pedro Eduardoen
dc.identifier.citationPandey, K., Pandey, B. D., Mallik, A. K., Acharya, J., Kato, K., Kaneko, O., et al. (2014). A New Molecular Surveillance System for Leishmaniasis. American Journal of Tropical Medicine and Hygiene, 13-0329.en
dc.description.abstractPresently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.en
dc.relation.ispartofseriesAmerican journal of tropical medicine and hygieneen
dc.rights© 2014 American Society of Tropical Medicine and Hygiene. This paper was published in American Journal of Tropical Medicine and Hygiene and is made available as an electronic reprint (preprint) with permission of American Society of Tropical Medicine and Hygiene. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleA new molecular surveillance system for Leishmaniasisen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.description.versionPublished versionen
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