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Title: Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in pseudomonas aeruginosa
Authors: Chua, Song Lin
Tan, Sean Yang-Yi
Rybtke, Morten Theil
Chen, Yicai
Rice, Scott A.
Kjelleberg, Staffan
Tolker-Nielsen, Tim
Yang, Liang
Givskov, Michael
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2013
Source: Chua, S. L., Tan, S. Y.-Y., Rybtke, M. T., Chen, Y., Rice, S. A., Kjelleberg, S., et al. (2013). Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in pseudomonas aeruginosa. Antimicrobial agents and chemotherapy, 57(5), 2066-2075.
Series/Report no.: Antimicrobial agents and chemotherapy
Abstract: Bis-(3′-5′)-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger that controls the lifestyles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used the expression of different reporters to show that planktonic cells, biofilm cells, and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in Pseudomonas aeruginosa. In accordance with this, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This finding contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.
ISSN: 0066-4804
DOI: 10.1128/AAC.02499-12
Rights: © 2013 American Society for Microbiology (ASM). This paper was published in Antimicrobial Agents and Chemotherapy and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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