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Title: Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
Authors: Dhand, Chetna
Balakrishnan, Yamini
Ong, Seow Theng
Dwivedi, Neeraj
Venugopal, Jayarama R.
Harini, Sriram
Leung, Chak Ming
Low, Kenny Zhi Wei
Loh, Xian Jun
Beuerman, Roger W.
Ramakrishna, Seeram
Verma, Navin Kumar
Lakshminarayanan, Rajamani
Keywords: DRNTU::Science::Medicine
Anti-infective Wound Dressing
Cyto-compatible Nanofibre
Issue Date: 2018
Source: Dhand, C., Balakrishnan, Y., Ong, S. T., Dwivedi, N., Venugopal, J. R., Harini, S., ... Lakshminarayanan, R. (2018). Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering. International Journal of Nanomedicine, Volume 13, 4473-4492. doi:10.2147/IJN.S159770
Series/Report no.: International Journal of Nanomedicine
Abstract: Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine.
ISSN: 1176-9114
DOI: 10.2147/IJN.S159770
Rights: © 2018 Dhand et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License ( By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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