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Title: Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication
Authors: Zhao, Yongqian
Soh, Tingjin Sherryl
Chan, Kitti Wing Ki
Fung, Sarah Suet Yin
Swaminathan, Kunchithapadam
Lim, Siew Pheng
Shi, Pei-Yong
Huber, Thomas
Lescar, Julien
Luo, Dahai
Vasudevan, Subhash G.
Issue Date: 2015
Source: Zhao, Y., Soh, T. S., Chan, K. W. K., Fung, S. S. Y., Swaminathan, K., Lim, S. P., et al. (2015). Flexibility of NS5 Methyltransferase-Polymerase Linker Region Is Essential for Dengue Virus Replication. Journal of Virology, 89(20), 10717-10721.
Series/Report no.: Journal of Virology
Abstract: We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In contrast, Pro substitution abolished virus replication by imposing rigidity in the linker and restricting NS5's conformational plasticity. Our biochemical and reverse genetics experiments demonstrate that NS5 utilizes conformational regulation to achieve optimum viral replication.
DOI: 10.1128/JVI.01239-15
Rights: © 2015 American Society for Microbiology. This paper was published in Journal of Virology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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