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Title: Chemical synthesis of circular proteins
Authors: Tam, James P.
Wong, Clarence T. T.
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2012
Source: Tam, J. P., & Wong, C. T. T. (2012). Chemical synthesis of circular proteins. Journal of biological chemistry, 287(32), 27020-27025.
Series/Report no.: Journal of biological chemistry
Abstract: Circular proteins, once thought to be rare, are now commonly found in plants. Their chemical synthesis, once thought to be difficult, is now readily achievable. The enabling methodology is largely due to the advances in entropic chemical ligation to overcome the entropy barrier in coupling the N- and C-terminal ends of large peptide segments for either intermolecular ligation or intramolecular ligation in end-to-end cyclization. Key elements of an entropic chemical ligation consist of a chemoselective capture step merging the N and C termini as a covalently linked O/S-ester intermediate to permit the subsequent step of an intramolecular O/S-N acyl shift to form an amide. Many ligation methods exploit the supernucleophilicity of a thiol side chain at the N terminus for the capture reaction, which makes cysteine-rich peptides ideal candidates for the entropy-driven macrocyclization. Advances in desulfurization and modification of the thiol-containing amino acids at the ligation sites to other amino acids add extra dimensions to the entropy-driven ligation methods. This minireview describes recent advances of entropy-driven ligation to prepare circular proteins with or without a cysteinyl side chain.
DOI: 10.1074/jbc.R111.323568
Rights: © 2012 The American Society for Biochemistry and Molecular Biology, Inc.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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