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Title: A small-molecule FRET reporter for the real-time visualization of cell-surface proteolytic enzyme functions
Authors: Mu, Jing
Liu, Fang
Rajab, Muhammad Shafiq
Shi, Meng
Li, Shuang
Goh, Chiching
Lu, Lei
Xu, Qing-Hua
Liu, Bin
Ng, Lai Guan
Xing, Bengang
Keywords: DRNTU::Science::Chemistry
Issue Date: 2014
Source: Mu, J., Liu, F., Rajab, M. S., Shi, M., Li, S., Goh, C., et al. (2014). A small-molecule FRET reporter for the real-time visualization of cell-surface proteolytic enzyme functions. Angewandte chemie international edition, 53(52), 14357-14362.
Series/Report no.: Angewandte chemie international edition
Abstract: Real-time imaging of cell-surface-associated proteolytic enzymes is critical to better understand their performances in both physiological and pathological processes. However, most current approaches are limited by their complexity and poor membrane-anchoring properties. Herein, we have designed and synthesized a unique small-molecule fluorescent probe, which combines the principles of passive exogenous membrane insertion and Förster resonance energy transfer (FRET) to image cell-surface-localized furin-like convertase activities. The membrane-associated furin-like enzymatic cleavage of the peptide probe leads to an increased fluorescence intensity which was mainly localized on the plasma membrane of the furin-expressed cells. This small-molecule fluorescent probe may serve as a unique and reliable reporter for real-time visualization of endogenous cell-surfaceassociated proteolytic furin-like enzyme functions in live cells and tissues using one-photon and two-photon microscopy.
ISSN: 1433-7851
DOI: 10.1002/anie.201407182
Rights: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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