Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/103995
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dc.contributor.authorChen, Chih-Haoen
dc.contributor.authorLiu, Jolene Mei-Junen
dc.contributor.authorShyu, Victor Bong-Hangen
dc.contributor.authorChen, Jyh-Pingen
dc.contributor.authorChou, Siaw Mengen
dc.contributor.authorChua, Chee Kaien
dc.date.accessioned2014-05-20T07:52:06Zen
dc.date.accessioned2019-12-06T21:24:11Z-
dc.date.available2014-05-20T07:52:06Zen
dc.date.available2019-12-06T21:24:11Z-
dc.date.copyright2014en
dc.date.issued2014en
dc.identifier.citationChen, C.-H., Liu, J. M.-J., Chua, C.-K., Chou, S. M., Shyu, V. B.-H., & Chen, J.-P. (2014). Cartilage tissue engineering with silk fibroin scaffolds fabricated by indirect additive manufacturing technology. Materials, 7(3), 2104-2119.en
dc.identifier.issn1996-1944en
dc.identifier.urihttps://hdl.handle.net/10356/103995-
dc.description.abstractAdvanced tissue engineering (TE) technology based on additive manufacturing (AM) can fabricate scaffolds with a three-dimensional (3D) environment suitable for cartilage regeneration. Specifically, AM technology may allow the incorporation of complex architectural features. The present study involves the fabrication of 3D TE scaffolds by an indirect AM approach using silk fibroin (SF). From scanning electron microscopic observations, the presence of micro-pores and interconnected channels within the scaffold could be verified, resulting in a TE scaffold with both micro- and macro-structural features. The intrinsic properties, such as the chemical structure and thermal characteristics of SF, were preserved after the indirect AM manufacturing process. In vitro cell culture within the SF scaffold using porcine articular chondrocytes showed a steady increase in cell numbers up to Day 14. The specific production (per cell basis) of the cartilage-specific extracellular matrix component (collagen Type II) was enhanced with culture time up to 12 weeks, indicating the re-differentiation of chondrocytes within the scaffold. Subcutaneous implantation of the scaffold-chondrocyte constructs in nude mice also confirmed the formation of ectopic cartilage by histological examination and immunostaining.en
dc.language.isoenen
dc.relation.ispartofseriesMaterialsen
dc.rights© 2014 The Author(s); licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).en
dc.subjectDRNTU::Engineering::Materialsen
dc.titleCartilage tissue engineering with silk fibroin scaffolds fabricated by indirect additive manufacturing technologyen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen
dc.identifier.doi10.3390/ma7032104en
dc.description.versionPublished versionen
item.grantfulltextopen-
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