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Title: Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells
Authors: Feng, Huixing
Li, Xi
Chan, Vincent
Chen, Wei Ning
Keywords: DRNTU::Science::Biological sciences::Biochemistry
Issue Date: 2014
Source: Feng, H., Li, X., Chan, V., & Chen, W. N. (2014). Proteomics Based Identification of Cell Migration Related Proteins in HBV Expressing HepG2 Cells. PLoS ONE, 9(4), e95621-.
Series/Report no.: PLoS ONE
Abstract: Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cell movement and kinetics changes due to the expression of HBV genome we have observed previously, here we further targeted to explore the specific changes of cellular proteins and potential intracellular protein interactions, which might provide more information of the potential cellular mechanism of the differentiated cell movements. Specific changes of a number of proteins were shown in global protein profiling in HepG2 cells expressing wildtype HBV, including cell migration related proteins, and interestingly the changes were found recovered by SH3 binding domain mutated HBV. The distinctive expressions of proteins were validated by Western blot analysis.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0095621
Rights: © 2014 Feng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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